Title
Electrospray Ionization–Ion Mobility Spectrometry Identified Monoclonal Antibodies that Bind Exclusively to Either the Monomeric or a Dimeric Form of Prostate Specific Antigen.
Funding Source
National Institutes of Health, U.S. Department of Energy
Grant Number
5SC1AI081660-04, 5G12RR026260-03, DE-SC0004959
Department
College of Pharmacy
Document Type
Article
Publication Date
7-17-2012
Abstract
Macroion mobility spectrometry was used to distinguish between a monoclonal antibody (clone M612165) that bound exclusively to monomeric prostate specific antigen and a different monoclonal antibody (clone M612166) that bound exclusively to a dimeric form of the antigen that only comprised 6.8% of the total protein. In the presence of excess antigen, the mobility spectrum of M612165 was replaced by a composite spectrum that represented a mixture of antibodies that included either one or two equivalents of the protein antigen. In similar circumstances, the mobility spectrum of M612166 was replaced by a composite spectrum that represented a mixture of antibodies that included either two or four equivalents of the protein antigen. When exposed to either of the two antibodies, the mobility spectrum of the prostate specific antigen showed a concomitant decrease in the monomeric antigen in one case and in the dimeric antigen in the other case. While sensitive kinetic exclusion assays demonstrated large differences in the antigen binding behavior of the two antibodies, these functional studies alone were insufficient to reveal the likely structural origins of the observed differences. Macroion mobility measurements were shown to be a useful and informative complement to functional studies in understanding complex macromolecular interactions.
Recommended Citation
Blake, Robert C. II and Blake, D. A., "Electrospray Ionization–Ion Mobility Spectrometry Identified Monoclonal Antibodies that Bind Exclusively to Either the Monomeric or a Dimeric Form of Prostate Specific Antigen." (2012). Faculty and Staff Publications. 254.
https://digitalcommons.xula.edu/fac_pub/254
Comments
DOI: 10.1021/ac301527v
PubMed ID: 22827589
This work was supported by Grants 5SC1AI081660-04 and 5G12RR026260-03 from the National Institutes of Health (RCB), and Grant DE-SC0004959 from the Office of Science within the U.S. Department of Energy (DAB).