Title

Rapidly Self-Renewing Human Multipotent Marrow Stromal Cells (hMSC) Express Sialyl Lewis X and Actively Adhere to Arterial Endothelium in a Chick Embryo Model System

Authors

H. McFerrin, Xavier University of LouisianaFollow
S. D. Olson, University of Texas Medical School at Houston
Miriam V. Gutschow, Stanford University
J. A. Semon, Tulane University School of Medicine, New Orleans
D. E. Sullivan, Tulane University School of Medicine
D. J. Prockop, Texas A and M Health Science Center, Temple, TX.

Funding Source

National Institute of General Medical Sciences Disparities, National Center for Research Resources, National Institutes of Health

Grant Number

P20GM103424, P20RR016456, P40 RR 17447

Department

Department of Biology

Document Type

Article

Publication Date

8-21-2014

Abstract

Background: There have been conflicting observations regarding the receptors utilized by human multipotent mesenchymal bone marrow stromal cells (hMSC) to adhere to endothelial cells (EC). To address the discrepancies, we performed experiments with cells prepared with a standardized, low-density protocol preserving a sub-population of small cells that are rapidly self-renewing. Methods: Sialyl Lewis X (SLeX) and α4 integrin expression were determined by flow cytometry. Fucosyltransferase expression was determined by quantitative realtime RT-PCR. Cell adhesion assays were carried out with a panel of endothelial cells from arteries, veins and the microvasculature in vitro. In vivo experiments were performed to determine single cell interactions in the chick embryo chorioallantoic membrane (CAM). The CAM is a well-characterized respiratory organ allowing for time-lapse image acquisition of large numbers of cells treated with blocking antibodies against adhesion molecules expressed on hMSC. Results: hMSC expressed α4 integrin, SLeX and fucosyltransferase 4 and adhered to human EC from arteries, veins and the microvasculature under static conditions in vitro. In vivo, hMSC rolled on and adhered to arterioles in the chick embryo CAM, whereas control melanoma cells embolized. Inhibition of α4 integrin and/or SLeX with blocking antibodies reduced rolling and adhesion in arterioles and increased embolism of hMSC. Conclusions: The results demonstrated that rapidly self-renewing hMSC were retained in the CAM because they rolled on and adhered to respiratory arteriolar EC in an α4 integrin- and SLeX-dependent manner. It is therefore important to select cells based on their cell adhesion receptor profile as well as size depending on the intended target of the cell and the injection route.

Comments

DOI: 10.1371/journal.pone.0105411

PubMed ID: 25144321

Recommended Citation

McFerrin, H.; Olson, S. D.; Gutschow, Miriam V.; Semon, J. A.; Sullivan, D. E.; and Prockop, D. J., "Rapidly Self-Renewing Human Multipotent Marrow Stromal Cells (hMSC) Express Sialyl Lewis X and Actively Adhere to Arterial Endothelium in a Chick Embryo Model System" (2014). Faculty and Staff Publications. 233.
https://digitalcommons.xula.edu/fac_pub/233