Title
A Human Apolipoprotein E Mimetic Peptide Effectively Inhibits HSV-1 TK-Positive and TK-Negative Acute Epithelial Keratitis in Rabbits.
Funding Source
National Eye Institute, Research to Prevent Blindness
Grant Number
R01 EY06311,F32EY016316, P30 EY002377
Department
Department of Biology
Document Type
Article
Publication Date
2-2009
Abstract
Purpose: To compare the antiviral effect of a peptide derived from human apolipoprotein E (1% apoEdp) with 1% trifluorothymidine against HSV-1 thymidine kinase (TK)-positive (HSV-1 McKrae) and with 3% foscarnet against HSV-1 TK-negative (KOS background) in the rabbit eye model of acute HSV-1 epithelial keratitis. Materials and Methods: Topical treatment began three days post-infection and continued for five days. Rabbits were treated 5 times per day with 1% apoEdp, 1% trifluorothymidine, 3% foscarnet, or PBS. Slit lamp examination was performed on a masked basis from post-infection days 3-10 to determine the severity of epithelial keratitis. Results: Topical treatment with 1% apoEdp was as effective as 1% trifluorothymidine against TK-positive and 3% foscarnet against TK-negative HSV-1 epithelial keratitis. Conclusions: ApoEdp 1% has efficacy for the topical treatment of TK-positive and TK-negative ocular herpes in the rabbit eye model. Thus, apoEdp has the potential to be used for treatment of keratitis caused by TK mutants.
Recommended Citation
Bhattacharjee, Partha S.; Neumann, D. M.; and Hill, J. M., "A Human Apolipoprotein E Mimetic Peptide Effectively Inhibits HSV-1 TK-Positive and TK-Negative Acute Epithelial Keratitis in Rabbits." (2009). Faculty and Staff Publications. 215.
https://digitalcommons.xula.edu/fac_pub/215
Comments
DOI: 10.1080/02713680802647662
PubMed ID: 19219680
Funding text
This work was supported in part by National Eye Institute Grants NEI R01 EY06311 (JMH), F32EY016316 (DMN), NIH NEI P30 EY002377 (LSU Eye Center Core Grant), Research to Prevent Blindness (RPB) Senior Scientific Award (JMH), LSUHSC Translational Research Initiative Grant (PSB), and an unrestricted departmental grant from Research to Prevent Blindness, New York, NY. This work was done at the LSU Eye Center, Louisiana State University Health Sciences Center, New Orleans, LA.