Title
New Zampanolide Mimics: Design, Synthesis, and Antiproliferative Evaluation.
Funding Source
Xavier University of Louisiana, National Institutes of Health, National Institute of General Medical Sciences
Grant Number
2G12MD007595, SC2GM121185
Department
Department of Chemistry
Document Type
Article
Publication Date
1-15-2020
Abstract
Zampanolide is a promising microtubule-stabilizing agent (MSA) with a unique chemical structure. It is superior to the current clinically used MSAs due to the covalent nature of its binding to β-tubulin and high cytotoxic potency toward multidrug-resistant cancer cells. However, its further development as a viable drug candidate is hindered by its limited availability. More importantly, conversion of its chemically fragile side chain into a stabilized bioisostere is envisioned to enable zampanolide to possess more drug-like properties. As part of our ongoing project aiming to develop its mimics with a stable side chain using straightforward synthetic approaches, 2-fluorobenzyl alcohol was designed as a bioisosteric surrogate for the side chain based on its binding conformation as confirmed by the X-ray structure of tubulin complexed with zampanolide. Two new zampanolide mimics with the newly designed side chain have been successfully synthesized through a 25-step chemical transformation for each. Yamaguchi esterification and intramolecular Horner–Wadsworth–Emmons condensation were used as key reactions to construct the lactone core. The chiral centers at C17 and C18 were introduced by the Sharpless asymmetric dihydroxylation. Our WST-1 cell proliferation assay data in both docetaxel-resistant and docetaxel-naive prostate cancer cell lines revealed that compound 6 is the optimal mimic and the newly designed side chain can serve as a bioisostere for the chemically fragile N-acetyl hemiaminal side chain in zampanolide.
Recommended Citation
Chen, G.; Jiang, Z.; Zhang, Q; Wang, Guangdi; and Chen, Q. H., "New Zampanolide Mimics: Design, Synthesis, and Antiproliferative Evaluation." (2020). Faculty and Staff Publications. 112.
https://digitalcommons.xula.edu/fac_pub/112
Comments
DOI: 10.1107/S0108270186095604
PubMed ID: 31952332
Funding text
Funding: This research was funded by the National Institutes of Health (National Institute of General Medical Sciences) under Award Number SC2GM121185 (Q. Chen). HRMS were supported by the National Institutes of Health RCMI program at Xavier University of Louisiana through Grant 2G12MD007595 (G. Wang).