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premenopausal African American and Hispanic women. Our group has taken an interest in nanosize membrane vesicles known as exosomes. We are particularly interested in the interaction that occurs between these exosomes and the tumor microenvironment, as it is believed that they promote proliferation, migration, and invasion. Therefore, we plan to evaluate the protein expression of TNFSF10 (TRAIL) in starved MCF-10A cells and the MCF-7 cell line. TRAIL is an apoptosis inducing ligand apart of the TNF superfamily. The MCF-7 cell line tends to be most insensitive to the TRAIL protein. In most cases cell lines are not sensitive to TRAIL because they lack the DR4 and/or DR5 receptors on their cell line surface or because they are deficient in caspase -8 and -3. The MCF-7 cell line contains both receptors, however they are deficient in both caspases. Therefore, we plan to evaluate the protein expression of TNFSF10 (TRAIL) in starved MCF-10A cells and the MCF-7 cell line. We plan to upregulate and down regulate TNFSF10 in the MCF-7 cell line to examine the functionality of the protein within the cell and tag our exosomes with TRAIL.
Xavier University of Louisiana
Exosomes Influence TRAIL Expression in MCF-7 Estrogen Sensitive Breast Cancer Cells
Roberson, Tresaundra M.; Coward, Jordon G.; Pekins, Kaitlyn S.; Banks, U’Ronnie; Johnson, Jasmine; and Lemieux, KiTani P., "Exosomes Influence TRAIL Expression in MCF-7 Estrogen Sensitive Breast Cancer Cells" (2020). Building Infrastructure Leading to Diversity (BUILD). 6.