A Human Apolipoprotein E Mimetic Peptide Effectively Inhibits HSV-1 TK-Positive and TK-Negative Acute Epithelial Keratitis in Rabbits.

Funding Source

National Eye Institute, Research to Prevent Blindness

Grant Number

R01 EY06311,F32EY016316, P30 EY002377


Department of Biology

Document Type


Publication Date



Purpose: To compare the antiviral effect of a peptide derived from human apolipoprotein E (1% apoEdp) with 1% trifluorothymidine against HSV-1 thymidine kinase (TK)-positive (HSV-1 McKrae) and with 3% foscarnet against HSV-1 TK-negative (KOS background) in the rabbit eye model of acute HSV-1 epithelial keratitis. Materials and Methods: Topical treatment began three days post-infection and continued for five days. Rabbits were treated 5 times per day with 1% apoEdp, 1% trifluorothymidine, 3% foscarnet, or PBS. Slit lamp examination was performed on a masked basis from post-infection days 3-10 to determine the severity of epithelial keratitis. Results: Topical treatment with 1% apoEdp was as effective as 1% trifluorothymidine against TK-positive and 3% foscarnet against TK-negative HSV-1 epithelial keratitis. Conclusions: ApoEdp 1% has efficacy for the topical treatment of TK-positive and TK-negative ocular herpes in the rabbit eye model. Thus, apoEdp has the potential to be used for treatment of keratitis caused by TK mutants.


DOI: 10.1080/02713680802647662

PubMed ID: 19219680

Funding text

This work was supported in part by National Eye Institute Grants NEI R01 EY06311 (JMH), F32EY016316 (DMN), NIH NEI P30 EY002377 (LSU Eye Center Core Grant), Research to Prevent Blindness (RPB) Senior Scientific Award (JMH), LSUHSC Translational Research Initiative Grant (PSB), and an unrestricted departmental grant from Research to Prevent Blindness, New York, NY. This work was done at the LSU Eye Center, Louisiana State University Health Sciences Center, New Orleans, LA.